Coding

Part:BBa_K3346000:Design

Designed by: Emily Laskey   Group: iGEM20_Rochester   (2020-08-17)


Siltuximab Heavy Variable Chain for IgG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used alternative codons in order to remove illegal restriction sites and optimize the sequence for all available BioBrick standards. We also performed codon optimization for an E. coli chassis using Reverse Translate Software available on The Sequence Manipulation Suite.


Source

This nucleotide sequence was converted from amino acid sequence and optimized for expression in E. coli using available online software.

Organism: chimeric (mouse and human)

References